BC10Bladder cancer-related protein BC10 |
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| SMART accession number: | SM01396
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| Description: |
This family consists of a series of short proteins of around 90 residues in length. The human protein O60629 or BC10 has been implicated in bladder cancer where the transcription of the gene coding for this protein is nearly completely abolished in highly invasive transitional cell carcinomas (TCCs) PMID:11920613. The protein is a small globular protein containing two transmembrane helices, and it is a multiply edited transcript. All the editing sites are found in either the 5-UTR or the N-terminal section of the protein, which is predicted to be outside the membrane. The three coding edits are all non-synonymous and predicted to encode exposed residues PMID:15797904. The function of this family is unknown. |
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| Interpro abstract (IPR009598): |
This family consists of a series of short proteins of around 90 residues in length. The human protein O60629 (or BC10) has been implicated in bladder cancer where the transcription of the gene coding for this protein is nearly completely abolished in highly invasive transitional cell carcinomas (TCCs) [ (PUBMED:15797904) ]. The protein is a small globular protein containing two transmembrane helices, and it is a multiply edited transcript. All the editing sites are found in either the 5'-UTR or the N-terminal section of the protein, which is predicted to be outside the membrane. The three coding edits are all non-synonymous and predicted to encode exposed residues [ (PUBMED:11920613) ]. The function of this family is unknown.
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| Family alignment: |
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There are 0 BC10 domains in 0 proteins in SMART's nrdb database.
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Literature (relevant references for this domain)
Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Gromova I, Gromov P, Celis JE
- bc10: A novel human bladder cancer-associated protein with a conserved genomicstructure downregulated in invasive cancer.
- Int J Cancer. 2002; 98: 539-46
- Display abstract
To identify novel genes that may be associated with the invasive phenotype ofbladder cancer, we compared the mRNA expression profiles of fresh noninvasive(grade II, Ta) and invasive (grade III, T2-T4) human transitional cell carcinomas(TCCs) by mRNA differential display. Using this approach, we isolated a novelgene, designated bc10 (bladder cancer, Mr 10 kDa) that was exclusively expressed in the noninvasive lesions as judged by reverse transcriptase polymerase chainreaction analysis of a panel of 30 grade II, Ta and grade III, T2-T4 TCCs. Thefull-length bc10 cDNA contains a complete open reading frame (ORF) of 263 bp and encodes a protein composed of 87 amino acids that has no homology to any of theknown protein families. Transient expression of bc10 cDNA in COS1 cells yielded aprimary translation product with an apparent Mr of 9.8 kDa and pI of 6.7, inagreement with the theoretical calculated value. Comparison of mouse and humanbc10 genomic loci revealed an intronless organization of the coding region inboth species as well as a highly conserved structure having 91% and 100% identityat the DNA (coding region) and protein levels, respectively. Southern analysisdid not reveal gross DNA rearrangements within the bc10 genomic locus in theinvasive tumors, implying that the differential expression of the gene mostlikely reflects alterations in messenger expression (transcription and/or mRNAdecay). The downregulation of this novel marker in invasive tumors suggests aputative role in bladder cancer progression.
Links (links to other resources describing this domain)
