CheW

Two component signalling adaptor domain

• SMART accession number: SM00260
• Description: -
• Interpro abstract (IPR002545):

  The CheW-like domain is an around 150-residue domain that is found in proteins involved in the two-component signaling systems regulating bacterial chemotaxis. Two components systems are composed of a receptor kinase, which monitors the environmental conditions and its substrate, the response regulator, which acts as a binary switch depending on the phosphorylation state. In Escherichia coli, the signal transduction pathway for chemotaxis consists of specialised membrane receptors, termed chemotaxis transducers; a CheA-CheY two-component system, which transmits the signal from transducers to flagellar motors; and a docking protein, CheW, which couples the CheA histidine kinase to transducers. Whereas CheW is only made of a CheW-like domain, CheA additionally contains an HPt domain and an histidine kinase domain. The CheW-like domain has been shown to mediate the interaction between CheA and the adaptor protein CheW. Some bacteria contain another bifunctional protein, CheV, consisting of an N- terminal CheW-like domain and a C-terminal response regulatory domain. Although its precise function in chemotaxis is unknown, CheV probably acts in adaptation to attractants [ (PUBMED:9989504) (PUBMED:11553614) (PUBMED:12511501) (PUBMED:11799399) ].

  The CheW-like domain is composed of two beta-sheet subdomains, each of which forms a loose five-stranded beta-barrel around an internal hydrophobic core. The interactions between the subdomains are contributed by a third hydrophobic core sandwiched between the two beta-sheet subdomains. The CheW-like structure is stabilised by extensive hydrophobic interactions [ (PUBMED:9989504) (PUBMED:11799399) ].

• GO process: chemotaxis (GO:0006935), signal transduction (GO:0007165)

There are 55999 CheW domains in 53950 proteins in SMART's nrdb database.

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
58.45	map02030	Bacterial chemotaxis - General
33.95	map02020	Two-component system - General
7.52	map03090	Type II secretion system
0.08	map00190	Oxidative phosphorylation

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with CheW domain which could be assigned to a KEGG orthologous group, and not all proteins containing CheW domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of CheW domains in PDB

PDB code	Title
1b3q	CRYSTAL STRUCTURE OF CHEA-289, A SIGNAL TRANSDUCING HISTIDINE KINASE
1k0s	Solution structure of the chemotaxis protein CheW from the thermophilic organism Thermotoga maritima
2ch4	Complex between Bacterial Chemotaxis histidine kinase CheA domains P4 and P5 and receptor-adaptor protein CheW
2ho9	Solution Structure of chemotaxi protein CheW from Escherichia coli
2qdl	Crystal structure of scaffolding protein TtCheW from Thermoanaerobacter tengcongensis
3ja6	3JA6
3ur1	The structure of a ternary complex between CheA domains P4 and P5 with CheW and with a truncated fragment of TM14, a chemoreceptor analog from Thermotoga maritima.
4jpb	The structure of a ternary complex between CheA domains P4 and P5 with CheW and with an unzipped fragment of TM14, a chemoreceptor analog from Thermotoga maritima.
4xfm	4XFM
4xgj	4XGJ

Links (links to other resources describing this domain)

• INTERPRO: IPR002545