Taxonomic distribution of proteins containing DeoC domains

The tree below includes only several representative species and genera. The complete taxonomic breakdown of all proteins containing DeoC domains can be accessed here. Click the counts or percentage values to display the corresponding proteins.

Deoxyribose 5-phosphate aldolase of Bacillus cereus: purification and properties.

Sgarrella F, Del Corso A, Tozzi MG, Camici M
Biochim Biophys Acta. 1992; 1118: 130-3

Deoxyribose 5-phosphate aldolase was purified 41 times from Bacillus cereusinduced by growth on deoxyribonucleosides. The purification procedure includesammonium sulphate fractionation, gel filtration on Sephadex G-100, ion-exchangechromatography on DEAE-Sephacel and preparative electrophoresis on 10%polyacrylamide gel. The enzyme is stable above pH 6.5, but is rapidly inactivatedby sulfhydryl reagents. Being insensitive to EDTA, it may be considered as aClass I aldolase. Among a number of compounds tested (including some carboxylicacids, free and phosphorylated pentoses, nucleotides and nucleosides), none hasbeen found to affect the enzyme activity. The enzyme appears to be dimeric, with a subunit Mr of 23,600. A Km of 4.4 x 10(-4) M was calculated for dRib 5-P.

Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, thestructural genes of the tagatose 6-phosphate pathway.

Rosey EL, Oskouian B, Stewart GC
J Bacteriol. 1991; 173: 5992-8

The nucleotide and deduced amino acid sequences of the lacA and lacB genes of theStaphylococcus aureus lactose operon (lacABCDFEG) are presented. The primarytranslation products are polypeptides of 142 (Mr = 15,425) and 171 (Mr = 18,953) amino acids, respectively. The lacABCD loci were shown to encode enzymes of thetagatose 6-phosphate pathway through both in vitro studies and complementationanalysis in Escherichia coli. A serum aldolase assay, modified to allow detectionof the tagatose 6-phosphate pathway enzymes utilizing galactose 6-phosphate orfructose phosphate analogs as substrate, is described. Expression of both lacAand lacB was required for galactose 6-phosphate isomerase activity. LacC (34 kDa)demonstrated tagatose 6-phosphate kinase activity and was found to sharesignificant homology with LacC from Lactococcus lactis and with both the minor6-phosphofructokinase (PfkB) and 1-phosphofructokinase (FruK) from E. coli.Detection of tagatose 1,6-bisphosphate aldolase activity was dependent onexpression of the 36-kDa protein specified by lacD. The LacD protein is highlyhomologous with LacD of L. lactis. Thus, the lacABCD genes comprise the tagatose 6-phosphate pathway and are cotranscribed with genes lacFEG, which specifyproteins for transport and cleavage of lactose in S. aureus.