emp24/gp25L/p24 family/GOLD
SMART accession number:SM01190
Description: Members of this family are implicated in bringing cargo forward from the ER and binding to coat proteins by their cytoplasmic domains. This domain corresponds closely to the beta-strand rich GOLD domain described in (PUBMED:12049664). The GOLD domain is always found combined with lipid- or membrane-association domains (PUBMED:12049664).
Interpro abstract (IPR009038):

The GOLD (for Golgi dynamics) domain is a protein module found in several eukaryotic Golgi and lipid-traffic proteins. It is typically between 90 and 150 amino acids long. Most of the size difference observed in the GOLD-domain superfamily is traceable to a single large low-complexity insert that is seen in some versions of the domain. With the exception of the p24 proteins, which have a simple architecture with the GOLD domain as their only globular domain, all other GOLD-domain proteins contain additional conserved globular domains. In these proteins, the GOLD domain co-occurs with lipid-, sterol- or fatty acid-binding domains such as PH, CRAL-TRIO, FYVE oxysterol binding- and acyl CoA-binding domains, suggesting that these proteins may interact with membranes. The GOLD domain can also be found associated with a RUN domain, which may have a role in the interaction of various proteins with cytoskeletal filaments. The GOLD domain is predicted to mediate diverse protein-protein interactions [(PUBMED:12049664)]. A secondary structure prediction for the GOLD domain reveals that it is likely to adopt a compact all-beta-fold structure with six to seven strands. Most of the sequence conservation is centred on the hydrophobic cores that support these predicted strands. The predicted secondary-structure elements and the size of the conserved core of the domain suggests that it may form a beta- sandwich fold with the strands arranged in two beta sheets stacked on each other [(PUBMED:12049664)].

Some proteins known to contain a GOLD domain are listed below:

  • Eukaryotic proteins of the p24 family.
  • Animal Sec14-like proteins. They are involved in secretion.
  • Human Golgi resident protein GCP60. It interacts with the Golgi integral membrane protein Giantin.
  • Yeast oxysterol-binding protein homologue 3 (OSH3).

Family alignment:
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There are 9982 EMP24_GP25L domains in 9973 proteins in SMART's nrdb database.

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