SMART: Pfam domain Cu_bind

Cu_bind_like

PFAM accession number:	PF02298
Interpro abstract (IPR003245):	Among the blue copper proteins with a single type I (or "blue") mononuclear copper site, the plant-specific phytocyanins constitute a distinct subfamily that can be further subdivided into the families of uclacyanins, stellacyanins, plantacyanins, and early nodulins. Stellacyanins have a blue copper coordinated by two His, one Cys and one Gln. In plantacyanins and uclacyanins, the ligands of the type-I Cu sites are two His, one Cys and one Met [ (PUBMED:9761472) (PUBMED:11085657) (PUBMED:15858169) (PUBMED:15631465) ]. Early nodulins lack amino acid residues that coordinate Cu, so they are believed to be involved in unknown processes without binding Cu [ (PUBMED:19897921) ]. Phytocyanins are found in chloropasts of higher plants. The phytocyanin domain has a core of seven polypeptide strands arranged as a beta-sandwich comprising two beta-sheets, beta-sheet I and beta-sheet II. Beta-sheet I consists of three beta-strands and beta-sheet II consists of four beta-strands. A disulfide bridge close the metal centre is characteristic for phytocyanins, in contrast to azurins, pseudoazurins, and plastocyanins, where a disulfide bond is located on the distal side of the beta-barrel. This disuldide bridge may play a crucial role in maintaining the tertiary structure of the protein and/or the formation of the copper binding centre because one of the His ligands of copper is followed directly by a bridging Cys residue [ (PUBMED:9761472) (PUBMED:11085657) (PUBMED:15858169) (PUBMED:15631465) ]. Some members of this family (P93328) may not bind copper due to the lack of key residues. Some proteins known to contain a phytocyanin domain are listed below: Cucumber basic protein (CBP). Spinach basic protein (SBP). Cucumber stellacyanin (CST). Zucchini mavicyanin. Horseradish umecyanin [ (PUBMED:5089608) (PUBMED:11945593) ]. Some of the proteins in this family are allergens. The allergens in this family include allergens with the following designations: Amb a 3.
GO function:	electron transfer activity (GO:0009055)

PFAM accession number:

PF02298

Interpro abstract (IPR003245):

Among the blue copper proteins with a single type I (or "blue") mononuclear copper site, the plant-specific phytocyanins constitute a distinct subfamily that can be further subdivided into the families of uclacyanins, stellacyanins, plantacyanins, and early nodulins. Stellacyanins have a blue copper coordinated by two His, one Cys and one Gln. In plantacyanins and uclacyanins, the ligands of the type-I Cu sites are two His, one Cys and one Met [ (PUBMED:9761472) (PUBMED:11085657) (PUBMED:15858169) (PUBMED:15631465) ]. Early nodulins lack amino acid residues that coordinate Cu, so they are believed to be involved in unknown processes without binding Cu [ (PUBMED:19897921) ]. Phytocyanins are found in chloropasts of higher plants.

The phytocyanin domain has a core of seven polypeptide strands arranged as a beta-sandwich comprising two beta-sheets, beta-sheet I and beta-sheet II. Beta-sheet I consists of three beta-strands and beta-sheet II consists of four beta-strands. A disulfide bridge close the metal centre is characteristic for phytocyanins, in contrast to azurins, pseudoazurins, and plastocyanins, where a disulfide bond is located on the distal side of the beta-barrel. This disuldide bridge may play a crucial role in maintaining the tertiary structure of the protein and/or the formation of the copper binding centre because one of the His ligands of copper is followed directly by a bridging Cys residue [ (PUBMED:9761472) (PUBMED:11085657) (PUBMED:15858169) (PUBMED:15631465) ]. Some members of this family (P93328) may not bind copper due to the lack of key residues.

Some proteins known to contain a phytocyanin domain are listed below:

Cucumber basic protein (CBP).
Spinach basic protein (SBP).
Cucumber stellacyanin (CST).
Zucchini mavicyanin.
Horseradish umecyanin [ (PUBMED:5089608) (PUBMED:11945593) ].

Some of the proteins in this family are allergens. The allergens in this family include allergens with the following designations: Amb a 3.

GO function:

electron transfer activity (GO:0009055)

This is a PFAM domain. For full annotation and more information, please see the PFAM entry Cu_bind_like