DUF3449

• PFAM accession number: PF11931
• Interpro abstract (IPR024598):

  This entry represents the C-terminal region of SF3a60 subunit (also known as SF3a3 and Spliceosome-associated protein 61 or Prp9 from yeast) from SF3a complex, found in eukaryotes. This domain has two conserved sequence motifs: PIP and CEICG. It contains a zinc-finger domain of the C2H2-type which might be important for RNA binding and protein-protein interactions with components of the SF3b complex [ (PUBMED:22314233) ].

  The mature U2 snRNP (small nuclear ribonucleoprotein particle) relies on the spliceosome assembly for its function, which involves the Splicing factor 3a (SF3a), an evolutionary eukaryotic conserved heterotrimeric complex, essential for pre-mRNA splicing [ (PUBMED:29360106) ]. This complex includes three subunits: SF3a60, SF3a66 and SF3a120 in the human complex, being Prp9, Prp11 and Prp21 the counterparts in Saccharomyces cerevisiae. SF3a60 possess a highly conserved C2H2-type zinc‐finger domain at the C‐terminal, while Prp9 has two such domains; SF3a66 and Prp11 contain one C2H2‐type zinc‐finger domain and SF3a120/Prp21 has two suppressor‐of‐white‐apricot and prp21/spp91 (SURP) domains, followed by a short segment of charged residues. The SURP2 domain of SF3a120/Prp21 has a role in SF3a60/Prp9 binding, however, SURP1 domain function is unknown. The yeast structure shows that Prp9 interacts with Prp21 via a bidentate‐binding mode, and Prp21 is wrapped around Prp11 [ (PUBMED:22314233) ].

• GO process: mRNA splicing, via spliceosome (GO:0000398)
• GO component: spliceosomal complex (GO:0005681)
• GO function: RNA binding (GO:0003723)

This is a PFAM domain. For full annotation and more information, please see the PFAM entry DUF3449