Glyco_hydro_48

• PFAM accession number: PF02011
• Interpro abstract (IPR000556):

  O-Glycosyl hydrolases ( EC 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ (PUBMED:7624375) (PUBMED:8535779) ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

  Glycoside hydrolase family 48 comprises enzymes with several known activities; endoglucanase ( EC 3.2.1.4 ); cellobiohydrolase ( EC 3.2.1.91 ).

  The largest cellulase gene sequenced to date is one of the cellulases (celA) from the genome of the thermophilic anaerobic bacterium Caldocellum saccharolyticum. The celA gene product is a polypeptide of 1751 amino acids; this has a multidomain structure comprising two catalytic domains and two cellulose-binding domains, linked by Pro-Thr-rich regions. The N-terminal domain encodes an endoglucanase activity on carboxymethylcellulose, consistent with its similarity to several endo-1, 4-beta-D-glucanase sequences. The C-terminal domain shows similarity to a cellulase from Clostridium thermocellum (CelS), which acts synergistically with a second component to hydrolyse crystalline cellulose [ (PUBMED:7612247) ].

• GO process: cellulose catabolic process (GO:0030245)
• GO function: hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553), cellulase activity (GO:0008810)

This is a PFAM domain. For full annotation and more information, please see the PFAM entry Glyco_hydro_48