Peptidase_M15

• PFAM accession number: PF01427
• Interpro abstract (IPR000755):

  This group of metallopeptidases belong to MEROPS peptidase family M15 (clan MD), subfamily M15D (vanX D-Ala-D-Ala dipeptidase).

  The D-alanyl-D-alanine dipeptidase enzyme from Enterococcus faecalis is also known as the vancomycin resistance protein VanX, and hydrolyses D-ala-D-ala. It has a 250-fold differential in catalytic efficiency for hydrolysis of D-ala-D-ala versus D-ala-D-lactate. The latter therefore remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the dipeptide D-ala-D-lactate rather than the dipeptide D-ala-D-ala, thereby preventing vancomycin from binding. The enzyme requires a metal cofactor, and is induced by vancomycin through regulation by VanS and VanR.

• GO process: proteolysis (GO:0006508)
• GO function: dipeptidase activity (GO:0016805), metallopeptidase activity (GO:0008237)

This is a PFAM domain. For full annotation and more information, please see the PFAM entry Peptidase_M15