SMART: Pfam domain TcdA

TcdA_TcdB

PFAM accession number:	PF12919
Interpro abstract (IPR024770):	This entry represents the N-terminal glucosyltransferase domain from TcdA and TcdB. It is also found in other toxins. The GTD of TcdB has been shown to glycosylate the host's RhoA protein [ (PUBMED:16054646) ]. Toxins A (TcdA) and B (TcdB) of Clostridium difficile belong to the family of clostridial glucosylating toxins. These toxins glucosylate small GTPases of Rho and Ras families, inhibiting the signalling and regulatory functions of these switch proteins. After receptor-binding, the toxins are endocytosed to reach acidic endosomal compartments from where the toxins are translocated into the cytosol [ (PUBMED:20498856) ]. TcdB has been shown to consist of a N-terminal glucosyltransferase domain (GTD), responsible for the biological effects of the toxin, a cysteine protease domain (CPD), responsible for autocatalytic cleavage, a hydrophobic region (HR), which has been suggested to be involved in toxin translocation, and a C-terminal repetitive domain involved in receptor binding. The pore-forming region of toxin B has been described to be in a region in the middle of the protein, within amino acid residues 830 and 990 [ (PUBMED:21231971) ].
GO function:	transferase activity, transferring glycosyl groups (GO:0016757)

PFAM accession number:

PF12919

Interpro abstract (IPR024770):

This entry represents the N-terminal glucosyltransferase domain from TcdA and TcdB. It is also found in other toxins. The GTD of TcdB has been shown to glycosylate the host's RhoA protein [ (PUBMED:16054646) ].

Toxins A (TcdA) and B (TcdB) of Clostridium difficile belong to the family of clostridial glucosylating toxins. These toxins glucosylate small GTPases of Rho and Ras families, inhibiting the signalling and regulatory functions of these switch proteins. After receptor-binding, the toxins are endocytosed to reach acidic endosomal compartments from where the toxins are translocated into the cytosol [ (PUBMED:20498856) ].

TcdB has been shown to consist of a N-terminal glucosyltransferase domain (GTD), responsible for the biological effects of the toxin, a cysteine protease domain (CPD), responsible for autocatalytic cleavage, a hydrophobic region (HR), which has been suggested to be involved in toxin translocation, and a C-terminal repetitive domain involved in receptor binding. The pore-forming region of toxin B has been described to be in a region in the middle of the protein, within amino acid residues 830 and 990 [ (PUBMED:21231971) ].

GO function:

transferase activity, transferring glycosyl groups (GO:0016757)

This is a PFAM domain. For full annotation and more information, please see the PFAM entry TcdA_TcdB