GGDEF

diguanylate cyclase

• SMART accession number: SM00267
• Description: Diguanylate cyclase, present in a variety of bacteria.

There are 8496 GGDEF domains in 8480 proteins in SMART's nrdb database.

Cellular role (predicted cellular role)

Cellular role: signalling

Literature (relevant references for this domain)

Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

• Hecht GB, Newton A
• Identification of a novel response regulator required for the swarmer-to-stalked-cell transition in Caulobacter crescentus.
• J Bacteriol. 1995; 177: 6223-9

The onset of motility late in the Caulobacter crescentus cell cycle depends on a signal transduction pathway mediated by the histidine kinase PleC and response regulator DivK. We now show that pleD, whose function is required for the subsequent loss of motility and stalk formation by the motile swarmer cell, encodes a 454-residue protein with tandem N-terminal response regulator domains D1 and D2 and a novel C-terminal GGDEF domain. The identification of pleD301, a semidominant suppressor of the pleC Mot phenotype, as a mutation predicted to result in a D-53-->G change in the D1 domain supports a role for phosphorylation in the PleD regulator. Disruptions constructed in the pleD open reading frame demonstrated that the gene is not essential and that the pleC phenotype can also be suppressed by a recessive, loss-of-function mutation. These results suggest that PleD is part of a signal transduction pathway controlling stalked-cell differentiation early in the C. crescentus cell cycle.

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
91.67	map02020	Two-component system - General
1.39	map02030	Bacterial chemotaxis - General
1.39	map00530	Aminosugars metabolism
1.39	map00562	Inositol phosphate metabolism
1.39	map00910	Nitrogen metabolism
1.39	map00340	Histidine metabolism
1.39	map00632	Benzoate degradation via CoA ligation

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with GGDEF domain which could be assigned to a KEGG orthologous group, and not all proteins containing GGDEF domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of GGDEF domains in PDB

PDB code	Title
1w25	Response regulator pled in complex with c-digmp
2v0n	Activated response regulator pled in complex with c-digmp and gtp-alpha-s
3bre	Crystal structure of p.aeruginosa pa3702
3ezu
3hva
3hvb
3hvw
3i5a
3i5b
3i5c
3icl
3ign