CUE

Domain that may be involved in binding ubiquitin-conjugating enzymes (UBCs)

• SMART accession number: SM00546
• Description: CUE domains also occur in two protein of the IL-1 signal transduction pathway, tollip and TAB2. Ponting (Biochem. J.) "Proteins of the Endoplasmic reticulum" (in press)
• Interpro abstract (IPR003892): This domain may be involved in binding ubiquitin-conjugating enzymes (UBCs). CUE domains also occur in two protein of the IL-1 signal transduction pathway, tollip and TAB2.

There are 311 CUE domains in 302 proteins in SMART's nrdb database.

Literature (relevant references for this domain)

Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

• Biederer T, Volkwein C, Sommer T
• Role of Cue1p in ubiquitination and degradation at the ER surface.
• Science. 1997; 278: 1806-9

Endoplasmic reticulum (ER) degradation of aberrant proteins is mediated by the ubiquitin-proteasome pathway. Here, a membrane-bound component of the ubiquitin system, Cue1p, was identified. It was shown to recruit the soluble ubiquitin-conjugating enzyme Ubc7p to the ER membrane. In the absence of Cue1p, unassembled and thus cytosolically mislocalized Ubc7p was unable to participate in ER degradation or in the turnover of soluble non-ER proteins. Moreover, ubiquitination by Cue1p-assembled Ubc7p and Ubc6p was a prerequisite for retrograde transport of lumenal substrates out of the ER, which suggests that ubiquitination is mechanistically integrated into the ER degradation process.

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
66.67	map04620	Toll-like receptor signaling pathway
29.17	map04010	MAPK signaling pathway
4.17	map00380	Tryptophan metabolism

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with CUE domain which could be assigned to a KEGG orthologous group, and not all proteins containing CUE domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of CUE domains in PDB

PDB code	Title
1mn3	Cue domain of yeast vps9p
1otr	Solution structure of a cue-ubiquitin complex
1p3q	Mechanism of ubiquitin recognition by the cue domain of vps9
1wgl	Solution structure of cue domain in the c-terminal of human toll-interacting protein (tollip)
2dae	Solution structure of the n-terminal cue domain in the human mitogen-activated protein kinase kinase kinase 7 interacting protein 2 (map3k7ip2)
2dhy	Solution structure of the cue domain in the human cue domain containing protein 1 (cuedc1)
2di0	Solution structure of the cue domain in the human activating signal cointegrator 1 complex subunit 2 (ascc2)
2ejs	Solution structure of ruh-076, a human cue domain
2ekf	Solution structure of ruh-075, a human cue domain

Links (links to other resources describing this domain)

• INTERPRO: IPR003892