Glyco_32

Glycosyl hydrolases family 32

• SMART accession number: SM00640
• Interpro abstract (IPR001362):

  O-Glycosyl hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families (PUBMED:7624375), (PUBMED:8535779). This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'.

  Glycoside hydrolase family 32 comprises enzymes with several known activities; invertase (EC 3.2.1.26); inulinase (EC 3.2.1.7); levanase (EC 3.2.1.65); exo-inulinase (EC 3.2.1.80); sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99); fructan:fructan 1-fructosyltransferase (EC 2.4.1.100).

• GO process: carbohydrate metabolic process (GO:0005975)
• GO function: hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553)

There are 658 Glyco_32 domains in 657 proteins in SMART's nrdb database.

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
51.37	map00500	Starch and sucrose metabolism
45.49	map00052	Galactose metabolism
2.35	map00530	Aminosugars metabolism
0.78	map00051	Fructose and mannose metabolism

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Glyco_32 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Glyco_32 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of Glyco_32 domains in PDB

PDB code	Title
1st8	Crystal structure of fructan 1-exohydrolase iia from cichorium intybus
1uyp	The three-dimensional structure of beta-fructosidase (invertase) from thermotoga maritima
1w2t	Beta-fructosidase from thermotoga maritima in complex with raffinose
1y4w	Crystal structure of exo-inulinase from aspergillus awamori in spacegroup p21
1y9g	Crystal structure of exo-inulinase from aspergillus awamori complexed with fructose
1y9m	Crystal structure of exo-inulinase from aspergillus awamori in spacegroup p212121
2ac1	Crystal structure of a cell-wall invertase from arabidopsis thaliana
2add	Crystal structure of fructan 1-exohydrolase iia from cichorium intybus in complex with sucrose
2ade	Crystal structure of fructan 1-exohydrolase iia from cichorium intybus in complex with fructose
2aey	Crystal structure of fructan 1-exohydrolase iia from cichorium intybus in complex with 2,5 dideoxy-2,5-immino-d- mannitol
2aez	Crystal structure of fructan 1-exohydrolase iia (e201q) from cichorium intybus in complex with 1-kestose
2oxb	Crystal structure of a cell-wall invertase (e203q) from arabidopsis thaliana in complex with sucrose
2qqu	Crystal structure of a cell-wall invertase (d239a) from arabidopsis thaliana in complex with sucrose
2qqv	Crystal structure of a cell-wall invertase (e203a) from arabidopsis thaliana in complex with sucrose
2qqw	Crystal structure of a cell-wall invertase (d23a) from arabidopsis thaliana in complex with sucrose

Links (links to other resources describing this domain)

• PFAM: Glyco_hydro_32
• INTERPRO: IPR001362