Glyco_25

Glycosyl hydrolases family 25

• SMART accession number: SM00641
• Interpro abstract (IPR002053):

  O-Glycosyl hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families (PUBMED:7624375), (PUBMED:8535779). This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'.

  Glycoside hydrolase family 25 comprises enzymes with only one known activity; lysozyme (EC 3.2.1.17).

  It has been shown (PUBMED:1916274), (PUBMED:1747104) that a number of cell-wall lytic enzymes are evolutionary related and can be classified into a single family. Two residues, an aspartate and a glutamate, have been shown (PUBMED:567645) to be important for the catalytic activity of the Charalopsis enzyme. These residues as well as some others in their vicinity are conserved in all proteins from this family.

• GO process: peptidoglycan catabolic process (GO:0009253), cell wall catabolic process (GO:0016998)
• GO function: lysozyme activity (GO:0003796)

There are 208 Glyco_25 domains in 195 proteins in SMART's nrdb database.

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
100.00	map00530	Aminosugars metabolism

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Glyco_25 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Glyco_25 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of Glyco_25 domains in PDB

PDB code	Title
1h09	Multimodular pneumococcal cell wall endolysin from phage cp-
1jfx	Crystal structure of the bacterial lysozyme from streptomyces coelicolor at 1.65 a resolution
1oba	Multimodular pneumococcal cell wall endolysin from phage cp- complexed with choline
2ixu	Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (wild-type endolysin)
2ixv	Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (e94q mutant)
2j8f	Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (e94q mutant in complex with a disaccharide-pentapeptide)
2j8g	Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (e94q mutant in complex with a tetrasaccharide-pentapeptide)
2wag

Links (links to other resources describing this domain)

• PFAM: Glyco_hydro_25
• INTERPRO: IPR002053