NPCBM

• SMART accession number: SM00776
• Description: This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.
• Interpro abstract (IPR013222):

  This entry represents a putative carbohydrate binding module (NPCBM) domain found at the N terminus of glycosyl hydrolase family 98 proteins [ (PUBMED:16212961) ].

  Proteins containing this domain include CpGH98 from C. perfringens. It specifically hydrolyses fucose-containing trisaccharides from the glycotopes of blood groups A and B and is implicated for the infectivity and virulence of this pathogen [ (PUBMED:15618227) ]. CpGH98 has a central TIM barrel catalytic domain, in which putative catalytic residues may be tentatively identified, and an N-terminal novel putative carbohydrate binding module (NPCBM) [ (PUBMED:15618227) ].

There are 1788 NPCBM domains in 1400 proteins in SMART's nrdb database.

Cellular role (predicted cellular role)

Cellular role: metabolism

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
25.00	map00600	Sphingolipid metabolism
25.00	map00603	Glycosphingolipid biosynthesis - globoseries
25.00	map00561	Glycerolipid metabolism
25.00	map00052	Galactose metabolism

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with NPCBM domain which could be assigned to a KEGG orthologous group, and not all proteins containing NPCBM domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of NPCBM domains in PDB

PDB code	Title
2vmg	The structure of CBM51 from Clostridium perfringens GH95 in complex with methyl-galactose
2vmh	The structure of CBM51 from Clostridium perfringens GH95
2vmi	The structure of seleno-methionine labelled CBM51 from Clostridium perfringens GH95
2vng	Family 51 carbohydrate binding module from a family 98 glycoside hydrolase produced by Clostridium perfringens in complex with blood group A-trisaccharide ligand.
2vno	Family 51 carbohydrate binding module from a family 98 glycoside hydrolase produced by Clostridium perfringens in complex with blood group B-trisaccharide ligand.
2vnr	Family 51 carbohydrate binding module from a family 98 glycoside hydrolase produced by Clostridium perfringens.
2ygl	The X-ray crystal structure of tandem CBM51 modules of Sp3GH98, the family 98 glycoside hydrolase from Streptococcus pneumoniae SP3-BS71
2ygm	The X-ray crystal structure of tandem CBM51 modules of Sp3GH98, the family 98 glycoside hydrolase from Streptococcus pneumoniae SP3-BS71, in complex with the blood group B antigen

Links (links to other resources describing this domain)

• INTERPRO: IPR013222
• PFAM: NPCBM