The domain within your query sequence starts at position 101 and ends at position 254; the E-value for the GAF domain shown below is 1.44e-3.



Domain present in phytochromes and cGMP-specific phosphodiesterases.
SMART accession number:SM00065
Description: Mutations within these domains in PDE6B result in autosomal recessive inheritance of retinitis pigmentosa.
Interpro abstract (IPR003018):

The GAF domain is named after some of the proteins it is found in, including cGMP-specific phosphodiesterases, adenylyl cyclases and FhlA. It is also found in and guanylyl cyclases and phytochromes [ (PUBMED:9433123) (PUBMED:20004158) ]. The structure of a GAF domain shows that the domain shares a similar fold with the PAS domain [ (PUBMED:11032796) ]. Adenylyl and guanylyl cyclases catalyse ATP and GTP to the second messengers cAMP and cGMP respectively, these products up-regulating catalytic activity by binding to the regulatory GAF domain(s). The opposite hydrolysis reaction is catalysed by phosphodiesterase. cGMP-dependent 3',5'-cyclic phosphodiesterase catalyses the conversion of guanosine 3',5'-cyclic phosphate to guanosine 5'-phosphate. Here too, cGMP regulates catalytic activity by GAF-domain binding. Phytochromes are regulatory photoreceptors in plants and bacteria which exist in two thermally stable states that are reversibly inter-convertible by light, the Pr state absorbs maximally in the red region of the spectrum, while the Pfr state absorbs maximally in the far-red region [ (PUBMED:20298248) ].

The GAF domain is also found in FhlA (formate hydrogen lyase transcriptional activator) and NifA, a transcriptional activator required for activation of most Nif operons, which are directly involved in nitrogen fixation. NifA interacts with sigma-54 [ (PUBMED:1404379) ].

GO function:protein binding (GO:0005515)
Family alignment:
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There are 232204 GAF domains in 203968 proteins in SMART's nrdb database.

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