The domain within your query sequence starts at position 18 and ends at position 61; the E-value for the Josephin domain shown below is 3.6e-11.
RQRLELCAVHALNNVLQEQLFSQEAADEICKRDSEHSLGSEGSS
Josephin |
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PFAM accession number: | PF02099 |
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Interpro abstract (IPR006155): | The Josephin domain is an eukaryotic protein module of about 180 residues, which occurs in stand-alone form in Josephin-like proteins, and as an amino- terminal domain associated with two or three copies of the ubiquitin- interacting motif (UIM) in ataxin 3-like proteins. Josephin domain-containing proteins function as de-ubiquitination enzymes [ (PUBMED:17696782) ]. Although it has originally been proposed that the Josephin domain could be an all-alpha helical domain distantly related to ENTH and VHS domains involved in membrane trafficking and regulatory adaptor function [ (PUBMED:12486728) ], it is now believed that it is a mainly alpha helical cysteine-protease domain predicted to be active against ubiquitin chains or related substrates [ (PUBMED:12944423) (PUBMED:14559776) ]. The Josephin domain contains two conserved histidines and one cysteine that is required for the ubiquitin protease activity [ (PUBMED:12944423) (PUBMED:14559776) ] and two ubiquitin-binding sites [ (PUBMED:19382171) ]. Some proteins known to contain a Josephin domain are:
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GO process: | protein deubiquitination (GO:0016579) |
GO function: | thiol-dependent ubiquitin-specific protease activity (GO:0004843) |
This is a PFAM domain. For full annotation and more information, please see the PFAM entry Josephin