The domain within your query sequence starts at position 256 and ends at position 450; the E-value for the d1tubb2 domain shown below is 1e-25.
LGDLMEHLVPHPEFKMLGVRNIPQMSAASLAYSAFTWAGLLKHLRQMLISSAKMEEGINW QVRPPLTGLPPIGKASAHKEHHFNTSLANLVVLRGREVHSADVEGFKDPALYTSWLEPVD AFSVWKTQRAFDKYEKSAALVSNSQLLVKPLDMIVGKAWNMFSSKAFIHQYTKFGMEEED FLDSFALLEQVVASY