The domain within your query sequence starts at position 1185 and ends at position 1205; the E-value for the WH2 domain shown below is 1.32e0.

LHSALMEAIHSSGGREKLRKT

WH2

Wiskott Aldrich syndrome homology region 2

• SMART accession number: SM00246
• Description: Wiskott Aldrich syndrome homology region 2 / actin-binding motif
• Interpro abstract (IPR003124):

  The WH2 (WASP-Homology 2, or Wiskott-Aldrich homology 2) domain is an ~18 amino acids actin-binding motif. This domain was first recognised as an essential element for the regulation of the cytoskeleton by the mammalian Wiskott-Aldrich syndrome protein (WASP) family. WH2 proteins occur in eukaryotes from yeast to mammals, in insect viruses, and in some bacteria. The WH2 domain is found as a modular part of larger proteins; it can be associated with the WH1 or EVH1 domain IPR000697 and with the CRIB domain IPR000095 and the WH2 domain can occur as a tandem repeat. The WH2 domain binds actin monomers and can facilitate the assembly of actin monomers into newly forming actin filaments [ (PUBMED:11434350) (PUBMED:11911886) ].

  Some proteins known to contain a WH2 domain:

  • Mammalian Wiskott-Aldrich syndrome protein (WASP), a possible regulator of lymphocyte and platelet function. Defects in WASP are the cause of Wiskott- Aldrich syndrome (WAS), an X-linked recessive disease characterised by immune dysregulation and microthrombocytopenia. WASP proteins bind the actin nucleating protein complex Arp2/3.
  • Mammalian N-WASP/WASL and WASF/SCAR/WAVE1-3, and yeast LAS17, which are also proteins from the WASP family that participate in the transduction of signals from the cell surface to the actin cytoskeleton.
  • WAS protein family homologue 1 (WASH1), acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerisation.
  • Baker's yeast Verprolin, a protein involved in cytoskeletal organisation and cellular growth.
  • Human WASP interacting protein (WASPIP/WIP), a WASP-, profilin- and actin-binding protein which induces actin polymerisation and redistribution.
  • Nuclear polyhedrosis virus (NPV) P61/78/83 capsid protein, which may be important for the persistence and survival of the virus.
  • Fruit fly Spir(e) protein, an actin nucleation factor involved in the development of oocytes and embryos. Spir is conserved among metazoans.
  • Mammalian metastasis suppressor 1 or Missing in Metastasis (MIM) protein, an actin-binding protein that may be related to cancer progression or tumor metastasis.
• GO function: actin binding (GO:0003779)

There are 10371 WH2 domains in 7489 proteins in SMART's nrdb database.

Cellular role (predicted cellular role)

Binding / catalysis: actin binding

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
53.12	map04520	Adherens junction
45.31	map04810	Regulation of actin cytoskeleton
1.56	map04320	Dorso-ventral axis formation

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with WH2 domain which could be assigned to a KEGG orthologous group, and not all proteins containing WH2 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of WH2 domains in PDB

PDB code	Title
2a3z	Ternary complex of the WH2 domain of WASP with Actin-DNAse I
2a40	Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
2a41	Ternary complex of the WH2 Domain of WIP with Actin-DNAse I
2d1k	Ternary complex of the WH2 domain of mim with actin-dnase I
2vcp	Crystal structure of N-Wasp VC domain in complex with skeletal actin
3m1f	Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL
3m3n	Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains
3mn5	Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
3mn7	Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
3p8c	Structure and Control of the Actin Regulatory WAVE Complex
3tu5	Actin complex with Gelsolin Segment 1 fused to Cobl segment
3ue5	ECP-cleaved Actin in complex with Spir domain D
4efh	Acanthamoeba Actin complex with Spir domain D
4jhd	Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu
4n78	The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton
4rwt	4RWT

Links (links to other resources describing this domain)

• INTERPRO: IPR003124