Blood coagulation factors V and VIII contain a C-terminal, twice repeated, domain of about 150 amino acids, which is called F5/8 type C, FA58C, or C1/C2- like domain. In the Dictyostelium discoideum (Slime mold) cell adhesion protein discoidin, a related domain, named discoidin I-like domain, DLD, or DS, has been found which shares a common C-terminal region of about 110 amino acids with the … expand

Blood coagulation factors V and VIII contain a C-terminal, twice repeated, domain of about 150 amino acids, which is called F5/8 type C, FA58C, or C1/C2- like domain. In the Dictyostelium discoideum (Slime mold) cell adhesion protein discoidin, a related domain, named discoidin I-like domain, DLD, or DS, has been found which shares a common C-terminal region of about 110 amino acids with the FA58C domain, but whose N-terminal 40 amino acids are much less conserved. Similar domains have been detected in other extracellular and membrane proteins [ PUBMED:3092220 PUBMED:8390675 PUBMED:8639264 ] In coagulation factors V and VIII the repeated domains compose part of a larger functional domain which promotes binding to anionic phospholipids on the surface of platelets and endothelial cells [ PUBMED:3125864 ]. The C-terminal domain of the second FA58C repeat (C2) of coagulation factor VIII has been shown to be responsible for phosphatidylserine-binding and essential for activity [ PUBMED:2110840 PUBMED:7515064 ]. It forms an amphipathic α-helix, which binds to the membrane [ PUBMED:7893714 ]. FA58C contains two conserved cysteines in most proteins, which link the extremities of the domain by a disulphide bond [ PUBMED:8504111 PUBMED:7613471 PUBMED:8856064 ]. A further disulphide bond is located near the C-terminal of the second FA58C domain in MFGM Q08431 [ PUBMED:8856064 ].

  +------------------------------------------------------------------------+ | +-+ | | | | | CxPLGxxQITASxxxxxRLxxxWxxxxWxxxxxxQGxxxxxxxxxxxxGNxxxxxxxxxxRxPxcxcLRxExGC'C': conserved cysteine involved in a disulphide bond.'c': cysteine involved in a disulphide bond in MFGM  Q08431.'x': any amino acid.upper case letters: conserved residues.