Taxonomic distribution of proteins containing AP4E_app_platf domains

The tree below includes only several representative species and genera. The complete taxonomic breakdown of all proteins containing AP4E_app_platf domains can be accessed here. Click the counts or percentage values to display the corresponding proteins.

Similar subunit interactions contribute to assembly of clathrin adaptor complexesand COPI complex: analysis using yeast three-hybrid system.

Takatsu H, Futatsumori M, Yoshino K, Yoshida Y, Shin HW, Nakayama K
Biochem Biophys Res Commun. 2001; 284: 1083-9

Clathrin adaptor protein (AP) complexes are heterotetramers composed of twolarge, one medium, and one small subunits. By exploiting the yeast three-hybridsystem, we have found that an interaction between the two large subunits of theAP-1 complex, gamma-adaptin and beta1-adaptin, is markedly enhanced in thepresence of the small subunit, sigma1. Similarly, two large subunits of the AP-4 complex, epsilon-adaptin and beta4-adaptin, are found to interact with each otheronly in the presence of the small subunit, sigma4. Furthermore, we have foundthat an interaction between two large subunits of the COPI F subcomplex,gamma-COP and beta-COP, is detectable only in the presence of zeta-COP. Becausethese COPI subunits have common ancestral origins to the corresponding APsubunits, these three-hybrid data, taken together with the previous two-hybriddata, suggest that the AP complexes and the COPI F subcomplex assemble by virtue of similar subunit interactions.