Taxonomic distribution of proteins containing FKS1_dom1 domains

The tree below includes only several representative species and genera. The complete taxonomic breakdown of all proteins containing FKS1_dom1 domains can be accessed here. Click the counts or percentage values to display the corresponding proteins.

Multiple functional domains of the yeast l,3-beta-glucan synthase subunit Fks1prevealed by quantitative phenotypic analysis of temperature-sensitive mutants.

Okada H, Abe M, Asakawa-Minemura M, Hirata A, Qadota H, Morishita K, Ohnuki S, Nogami S, Ohya Y
Genetics. 2010; 184: 1013-24

The main filamentous structural component of the cell wall of the yeastSaccharomyces cerevisiae is 1,3-beta-glucan, which is synthesized by a plasmamembrane-localized enzyme called 1,3-beta-glucan synthase (GS). Here we analyzed the quantitative cell morphology and biochemical properties of 10 differenttemperature-sensitive mutants of FKS1, a putative catalytic subunit of GS. Tountangle their pleiotropic phenotypes, the mutants were classified into threefunctional groups. In the first group, mutants fail to synthesize 1,3-beta-glucanat the proper subcellular location, although GS activity is normal in vitro. Inthe second group, mutants have normal 1,3-beta-glucan content but are defectivein polarized growth and endocytosis. In the third group, mutations in theputative catalytic domain of Fks1p result in a loss of the catalytic activity of GS. The differences among the three groups suggest that Fks1p consists ofmultiple domains that are required for cell wall construction and cellularmorphogenesis.