Taxonomic distribution of proteins containing LAG1_DNAbind domains

The tree below includes only several representative species and genera. The complete taxonomic breakdown of all proteins containing LAG1_DNAbind domains can be accessed here. Click the counts or percentage values to display the corresponding proteins.

Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA.

Kovall RA, Hendrickson WA
EMBO J. 2004; 23: 3441-51

Notch signaling is a conserved pathway of communication between neighboring cellsthat results in cell fate specification, and CSL is the universal transcriptionaleffector of Notch signaling. The Notch intracellular domain translocates to thenucleus after proteolytic release upon Notch extracellular engagement, and there it displaces corepressors from DNA-bound CSL and recruits activators of Notchtarget genes. Here we report the 2.85 A crystal structure of CSL with a targetDNA. CSL comprises three structurally integrated domains: its amino (NTD)- andcarboxy (CTD)-terminal domains are strikingly similar to those of Reltranscription factors, but a surprising beta-trefoil domain (BTD) is insertedbetween them. CSL-bound DNA is recognized specifically by conserved residues fromNTD and BTD. A hydrophobic pocket on BTD is identified as the likely site ofNotch interaction with CSL, which has functional implications for the mechanismof Notch signaling.