This entry represents a zinc ribbon domain found at the terminal of DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase III subunit RPC10, archaeal Transcription factor S and similar sequences.
RPB9 is the core component of RNA polymerase II, the central component of the basal RNA polymerase II transcription machinery. POLR2I/RPB9 is part of the upper jaw surrounding … expand
This entry represents a zinc ribbon domain found at the terminal of DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase III subunit RPC10, archaeal Transcription factor S and similar sequences.
RPB9 is the core component of RNA polymerase II, the central component of the basal RNA polymerase II transcription machinery. POLR2I/RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template [ PUBMED:30190596 ]. DNA-directed RNA polymerase III subunit RPC10 is a core component of RNA polymerase III (Pol III) which synthesises small non-coding RNAs using the four ribonucleoside triphosphates as substrates [ PUBMED:30584594 PUBMED:33335104 PUBMED:33558764 ].
Proteins containing this domain also include transcription factor S (TFS), a protein related in size and sequence to DNA-directed RNA polymerase subunit M, and in sequence and function to the much larger eukaryotic transcription factor IIS (TFIIS). Although originally suggested to be a subunit of the archaeal RNA polymerase (known as archaeal DNA-directed RNA polymerase subunit M), it elutes separately from active polymerase in gel filtration experiments and acts, like TFIIs, as an induction factor for RNA cleavage by RNA polymerase [ PUBMED:10777522 PUBMED:15130130 ]. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides. It has recently been shown [ PUBMED:8265347 PUBMED:8417319 ] that small subunits of about 15kDa, found in polymerase types I and II, are highly conserved. These proteins contain a probable zinc finger in their N-terminal region and a C-terminal zinc ribbon domain (see IPR001222 )
DNA-directed RNA polymerases EC:2.7.7.6 (also known as DNA-dependent RNA polymerases) are responsible for the polymerisation of ribonucleotides into a sequence complementary to the template DNA. In eukaryotes, there are three different forms of DNA-directed RNA polymerases transcribing different sets of genes. Most RNA polymerases are multimeric enzymes and are composed of a variable number of subunits. The core RNA polymerase complex consists of five subunits (two alpha, one beta, one beta-prime and one omega) and is sufficient for transcription elongation and termination but is unable to initiate transcription. Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme [ PUBMED:3052291 ]. The core RNA polymerase complex forms a "crab claw"-like structure with an internal channel running along the full length [ PUBMED:10499798 ]. The key functional sites of the enzyme, as defined by mutational and cross-linking analysis, are located on the inner wall of this channel.
RNA synthesis follows after the attachment of RNA polymerase to a specific site, the promoter, on the template DNA strand. The RNA synthesis process continues until a termination sequence is reached. The RNA product, which is synthesised in the 5' to 3' direction, is known as the primary transcript.
Eukaryotic nuclei contain three distinct types of RNA polymerases that differ in the RNA they synthesise:
- RNA polymerase I: located in the nucleoli, synthesises precursors of most ribosomal RNAs.
- RNA polymerase II: occurs in the nucleoplasm, synthesises mRNA precursors.
- RNA polymerase III: also occurs in the nucleoplasm, synthesises the precursors of 5S ribosomal RNA, the tRNAs, and a variety of other small nuclear and cytosolic RNAs.
Eukaryotic cells are also known to contain separate mitochondrial and chloroplast RNA polymerases. Eukaryotic RNA polymerases, whose molecular masses vary in size from 500 to 700kDa, contain two non-identical large (>100kDa) subunits and an array of up to 12 different small (less than 50kDa) subunits.
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