Taxonomic distribution of proteins containing RTC4 domains

The tree below includes only several representative species and genera. The complete taxonomic breakdown of all proteins containing RTC4 domains can be accessed here. Click the counts or percentage values to display the corresponding proteins.

A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae.

Addinall SG, Downey M, Yu M, Zubko MK, Dewar J, Leake A, Hallinan J, Shaw O, James K, Wilkinson DJ, Wipat A, Durocher D, Lydall D
Genetics. 2008; 180: 2251-66

In Saccharomyces cerevisiae, Cdc13 binds telomeric DNA to recruit telomerase and to "cap" chromosome ends. In temperature-sensitive cdc13-1 mutants telomeric DNA is degraded and cell-cycle progression is inhibited. To identify novel proteinsand pathways that cap telomeres, or that respond to uncapped telomeres, wecombined cdc13-1 with the yeast gene deletion collection and used high-throughputspot-test assays to measure growth. We identified 369 gene deletions, in eightdifferent phenotypic classes, that reproducibly demonstrated subtle geneticinteractions with the cdc13-1 mutation. As expected, we identified DNA damagecheckpoint, nonsense-mediated decay and telomerase components in our screen.However, we also identified genes affecting casein kinase II activity, cellpolarity, mRNA degradation, mitochondrial function, phosphate transport, irontransport, protein degradation, and other functions. We also identified a number of genes of previously unknown function that we term RTC, for restriction oftelomere capping, or MTC, for maintenance of telomere capping. It seems likelythat many of the newly identified pathways/processes that affect growth ofbudding yeast cdc13-1 mutants will play evolutionarily conserved roles attelomeres. The high-throughput spot-testing approach that we describe isgenerally applicable and could aid in understanding other aspects of eukaryoticcell biology.