NORMAL GENOMIC

• Iobbi-Nivol C, Pommier J, Simala-Grant J, Mejean V, Giordano G
• High substrate specificity and induction characteristics of trimethylamine-N-oxide reductase of Escherichia coli.
• Biochim Biophys Acta. 1996; 1294: 77-82
• Display abstract

• Using a wide variety of N- and S-oxide compounds we have shown by kinetic analysis that only two N-oxides, trimethylamine-N-oxide and 4-methylmorpholine-N-oxide, can be considered good substrates for trimethylamine-N-oxide (TMAO) reductase on the basis of their kcat/Km ratio. This result demonstrates that TMAO reductase possesses a high substrate specificity. Induction of the torCAD operon using the same S- and N-oxide compounds was also analyzed. We demonstrate that there is no correlation between the ability for a compound to be reduced by TMAO reductase and to induce TMAO reductase synthesis.