O-Glycosyl hydrolases ( EC:3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ … expand

O-Glycosyl hydrolases ( EC:3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ PUBMED:7624375 PUBMED:8535779 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glycoside hydrolase family 10 comprises enzymes with a number of known activities; xylanase ( EC:3.2.1.8 ); endo-1,3-beta-xylanase ( EC:3.2.1.32 ); cellobiohydrolase ( EC:3.2.1.91 ). These enzymes were formerly known as cellulase family F.

The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases ( EC:3.2.1.4 ), cellobiohydrolases ( EC:3.2.1.91 ) (exoglucanases), or xylanases ( EC:3.2.1.8 ) [ PUBMED:2252383 PUBMED:1886523 ]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family F [ PUBMED:2806912 ] or as the glycosyl hydrolases family 10 [ PUBMED:1747104 ].

The overall structure of the GH10 domain corresponds to an eightfold α/β-barrel (TIM-barrel) with a typical deep groove in the centre, allowing an 'endo' type of action on the large polysaccharide backbone [ PUBMED:17642511 ], [ PUBMED:23508990 ].