The domain within your query sequence starts at position 284 and ends at position 381; the E-value for the Fic domain shown below is 1.8e-29.
SVTMDDMLEIHRRVLGYVDPVEAGRFRRTQVLVGHHIPPHPRDVEKQMQEFTQWLNSEDA MNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLIL
Fic |
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PFAM accession number: | PF02661 |
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Interpro abstract (IPR003812): | This globular domain is named fido after the Fic and Doc proteins where it is found. It is approximately 125 to 150 residues long, and is present in proteins from all kingdoms of life [ (PUBMED:14659018) (PUBMED:18757857) (PUBMED:19127588) (PUBMED:19503829) ], including:
The fido domain of Vibrio VopS covalently modifies Rho GTPase threonine with AMP to inhibit downstream signaling events in host cells. The AMPylation activity extends to a eukaryotic fido domain in Drosophila fic homologue CG9523. AMPylation represents a newly discovered posttranslational modification used to stably modify proteins with AMP. This signaling mechanism is predicted to be functionally similar to other posttranslation modifications such as phosphorylation, SUMOylation or acetylation, because the added moiety changes the activity of the modified protein. The covalent attachment of AMP by a phosphodiester bond is predicted to be reversible and is bulky enough to provide a docking site for a putative AMP binding domain [ (PUBMED:19503829) ]. The fido domain contains a central motif conserved in most sequences (H-x-F-x-[DE]-[AG]-N-[GK]-R), with the motif His contributing to fic AMPylation. The fido domain adopts an alpha-helical fold, arranged as a six-helix up and down bundle [ (PUBMED:18757857) (PUBMED:19127588) (PUBMED:19503829) ]. |
This is a PFAM domain. For full annotation and more information, please see the PFAM entry Fic