The domain within your query sequence starts at position 284 and ends at position 398; the E-value for the RHOD domain shown below is 3.71e-21.

All catalytic sites are present in this domain. Check the literature (PubMed 20251047 ) for details.

AALLSGKFQSVIERFYIIDCRYPYEYLGGHILGALNLHSQKELHEFFLRKPVVPLDIQKR
VIIVFLCEFSSERGPRMCRSLREKDRALNQYPALYYPELYILKGGYRDFFPEYME

RHOD

Rhodanese Homology Domain

• SMART accession number: SM00450
• Description: An alpha beta fold found duplicated in the Rhodanese protein. The the Cysteine containing enzymatically active version of the domain is also found in the CDC25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and stress proteins such as Senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. Inactive versions with a loss of the cysteine are also seen in Dual specificity phosphatases, ubiquitin hydrolases from yeast and in sulfuryltransferases. These are likely to play a role in protein interactions.
• Interpro abstract (IPR001763):

  Rhodanese, a sulphurtransferase involved in cyanide detoxification (see IPR001307 ) shares evolutionary relationship with a large family of proteins [ (PUBMED:9733650) ], including

  • Cdc25 phosphatase catalytic domain.
  • non-catalytic domains of eukaryotic dual-specificity MAPK-phosphatases.
  • non-catalytic domains of yeast PTP-type MAPK-phosphatases.
  • non-catalytic domains of yeast Ubp4, Ubp5, Ubp7.
  • non-catalytic domains of mammalian Ubp-Y.
  • Drosophila heat shock protein HSP-67BB.
  • several bacterial cold-shock and phage shock proteins.
  • plant senescence associated proteins.
  • catalytic and non-catalytic domains of rhodanese (see IPR001307 ).

  Rhodanese has an internal duplication. This domain is found as a single copy in other proteins, including phosphatases and ubiquitin C-terminal hydrolases [ (PUBMED:8702871) ].

There are 171652 RHOD domains in 136038 proteins in SMART's nrdb database.

Literature (relevant references for this domain)

Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

• Hofmann K, Bucher P, Kajava AV
• A model of Cdc25 phosphatase catalytic domain and Cdk-interaction surface based on the presence of a rhodanese homology domain.
• J Mol Biol. 1998; 282: 195-208

Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression. The catalytic domain of this dual-specificity phosphatase has recently been mapped to the 180 most C-terminal amino acids. Apart from a CX3R motif, which is present at the active site of all known tyrosine phosphatases, Cdc25 does not share any obvious sequence similarity with any of those enzymes. Until very recently, the Cdc25 family was the only subfamily of tyrosine phosphates for which no three-dimensional structural data were available. Using the generalized profile technique, a sensitive method for sequence database searches, we found an extended and highly significant sequence similarity between the Cdc25 catalytic domain and similarly sized regions in other proteins: the non-catalytic domain of two distinct families of MAP-kinase phosphates, the non-catalytic domain of several ubiquitin protein hydrolases, the N and C-terminal domain of rhodanese, and a large and heterogeneous groups of stress-response proteins from all phyla. The relationship of Cdc25 to the structurally well-characterized rhodanese spans the entire catalytic domain and served as template for a structural model for human Cdc25a, which is fundamentally different from previously suggested models for Cdc25 catalytic domain organization. The surface positioning of subfamily-specific conserved residues allows us to predict the sites of interaction with Cdk2, a physiological target of Cdc25a. Based on the results of this analysis, we also predict that the budding yeast arsenate resistance protein Acr2 and the ORF Ygr203w encode protein phosphatases with catalytic properties similar to that of the Cdc25 family. Recent determination of the crystal structure of the Cdc25a catalytic domain supports the validity of the model and demonstrates the power of the generalized sequence profile technique in homology-based modeling of the three-dimensional structure of a protein having a weak but significant sequence similarity with a structurally characterized protein.

Metabolism (metabolic pathways involving proteins which contain this domain)

% proteins involved	KEGG pathway ID	Description
29.39	map00272	Cysteine metabolism
26.34	map04010	MAPK signaling pathway
9.54	map00790	Folate biosynthesis
8.40	map00620	Pyruvate metabolism
5.73	map04110	Cell cycle
5.73	map04914	Progesterone-mediated oocyte maturation
3.44	map00190	Oxidative phosphorylation
3.05	map00730	Thiamine metabolism
1.91	map04111	Cell cycle - yeast
1.15	map00260	Glycine, serine and threonine metabolism
1.15	map00910	Nitrogen metabolism
1.15	map00564	Glycerophospholipid metabolism
0.76	map00450	Selenoamino acid metabolism
0.76	map00920	Sulfur metabolism
0.38	map00252	Alanine and aspartate metabolism
0.38	map00500	Starch and sucrose metabolism
0.38	map00460	Cyanoamino acid metabolism
0.38	map00271	Methionine metabolism

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with RHOD domain which could be assigned to a KEGG orthologous group, and not all proteins containing RHOD domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

Structure (3D structures containing this domain)

3D Structures of RHOD domains in PDB

PDB code	Title
1boh	SULFUR-SUBSTITUTED RHODANESE (ORTHORHOMBIC FORM)
1boi	N-TERMINALLY TRUNCATED RHODANESE
1c25	HUMAN CDC25A CATALYTIC DOMAIN
1cwr	HUMAN CDC25B CATALYTIC DOMAIN WITHOUT ION IN CATALYTIC SITE
1cws	HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE
1cwt	HUMAN CDC25B CATALYTIC DOMAIN WITH METHYL MERCURY
1dp2	CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RHODANESE AND LIPOATE
1e0c	SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII
1gmx	Escherichia coli GlpE sulfurtransferase
1gn0	Escherichia coli GlpE sulfurtransferase soaked with KCN
1h4k	Sulfurtransferase from Azotobacter vinelandii in complex with hypophosphite
1h4m	Sulfurtransferase from Azotobacter vinelandii in complex with phosphate
1hzm	STRUCTURE OF ERK2 BINDING DOMAIN OF MAPK PHOSPHATASE MKP-3: STRUCTURAL INSIGHTS INTO MKP-3 ACTIVATION BY ERK2
1okg	3-mercaptopyruvate sulfurtransferase from Leishmania major
1orb	ACTIVE SITE STRUCTURAL FEATURES FOR CHEMICALLY MODIFIED FORMS OF RHODANESE
1qb0	HUMAN CDC25B CATALYTIC DOMAIN
1qxn	Solution Structure of the 30 kDa Polysulfide-sulfur Transferase Homodimer from Wolinella Succinogenes
1rhd	STRUCTURE OF BOVINE LIVER RHODANESE. I. STRUCTURE DETERMINATION AT 2.5 ANGSTROMS RESOLUTION AND A COMPARISON OF THE CONFORMATION AND SEQUENCE OF ITS TWO DOMAINS
1rhs	SULFUR-SUBSTITUTED RHODANESE
1t3k	NMR structure of a CDC25-like dual-specificity tyrosine phosphatase of Arabidopsis thaliana
1tq1	Solution structure of At5g66040, a putative protein from Arabidosis Thaliana
1uar	Crystal structure of Rhodanese from Thermus thermophilus HB8
1urh	The ""Rhodanese"" fold and catalytic mechanism of 3-mercaptopyruvate sulfotransferases: Crystal structure of SseA from Escherichia coli
1whb	Solution structure of the Rhodanese-like domain in human ubiquitin specific protease 8 (UBP8)
1wv9	Crystal Structure of Rhodanese Homolog TT1651 from an Extremely Thermophilic Bacterium Thermus thermophilus HB8
1ym9	Crystal structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfinic form
1ymd	Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfonic form
1ymk	Crystal Structure of the CDC25B phosphatase catalytic domain in the apo form
1yml	Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfenic form
1ys0	Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the disulfide form
1yt8	Crystal Structure of Thiosulfate sulfurtransferase from Pseudomonas aeruginosa
2a2k	Crystal Structure of an active site mutant, C473S, of Cdc25B Phosphatase Catalytic Domain
2eg3	Crystal Structure of Probable Thiosulfate Sulfurtransferase
2eg4	Crystal Structure of Probable Thiosulfate Sulfurtransferase
2fsx	Crystal structure of Rv0390 from M. tuberculosis
2gwf	Structure of a USP8-NRDP1 complex
2hhg	Structure of Protein of Unknown Function RPA3614, Possible Tyrosine Phosphatase, from Rhodopseudomonas palustris CGA009
2ifd	Crystal structure of a remote binding site mutant, R492L, of CDC25B Phosphatase catalytic domain
2ifv	Crystal structure of an active site mutant, C473D, of CDC25B phosphatase catalytic domain
2j6p	Structure of As-Sb reductase from Leishmania major
2jtq	Rhodanese from E.coli
2jtr	rhodanese persulfide from E. coli
2jts	rhodanese with anions from E. coli
2k0z	Solution NMR structure of protein hp1203 from Helicobacter pylori 26695. Northeast Structural Genomics Consortium (NESG) target PT1/Ontario Center for Structural Proteomics target hp1203
2kl3	Solution NMR structure of the Rhodanese-like domain from Anabaena sp Alr3790 protein. Northeast Structural Genomics Consortium Target NsR437A
2moi	2MOI
2mol	2MOL
2mrm	2MRM
2ora	RHODANESE (THIOSULFATE: CYANIDE SULFURTRANSFERASE)
2ouc	Crystal structure of the MAP kinase binding domain of MKP5
2uzq	Protein Phosphatase, New Crystal Form
2vsw	The structure of the rhodanese domain of the human dual specificity phosphatase 16
2wlr	Putative thiosulfate sulfurtransferase YnjE
2wlx	Putative thiosulfate sulfurtransferase YnjE
3aax	Crystal structure of probable thiosulfate sulfurtransferase cysa3 (RV3117) from Mycobacterium tuberculosis: monoclinic FORM
3aay	Crystal structure of probable thiosulfate sulfurtransferase CYSA3 (RV3117) from Mycobacterium tuberculosis: orthorhombic form
3d1p	Atomic resolution structure of uncharacterized protein from Saccharomyces cerevisiae
3dtt	Crystal structure of a putative f420 dependent nadp-reductase (arth_0613) from arthrobacter sp. fb24 at 1.70 A resolution
3f4a	Structure of Ygr203w, a yeast protein tyrosine phosphatase of the Rhodanese family
3flh	Crystal structure of lp_1913 protein from Lactobacillus plantarum,Northeast Structural Genomics Consortium Target LpR140B
3fnj	Crystal structure of the full-length lp_1913 protein from Lactobacillus plantarum, Northeast Structural Genomics Consortium Target LpR140
3foj	Crystal Structure of SSP1007 From Staphylococcus saprophyticus subsp. saprophyticus. Northeast Structural Genomics Target SyR101A.
3fs5	Crystal structure of Saccharomyces cerevisiae Ygr203w, a homolog of single-domain rhodanese and Cdc25 phosphatase catalytic domain
3g5j	Crystal structure of N-terminal domain of putative ATP/GTP binding protein from Clostridium difficile 630
3gk5	Crystal structure of rhodanese-related protein (TVG0868615) from Thermoplasma volcanium, Northeast Structural Genomics Consortium Target TvR109A
3hix	Crystal Structure of the Rhodanese_3 like domain from Anabaena sp Alr3790 protein. Northeast Structural Genomics Consortium Target NsR437i
3hwi	Crystal structure of probable thiosulfate sulfurtransferase Cysa2 (Rhodanese-like protein) from Mycobacterium tuberculosis
3hzu	Crystal structure of probable thiosulfate sulfurtransferase SSEA (rhodanese) from Mycobacterium tuberculosis
3i2v	Crystal structure of human MOCS3 rhodanese-like domain
3i3u	Crystal structure of lp_1913 protein from lactobacillus plantarum, northeast structural genomics Consortium target lpr140a
3icr	Crystal structure of oxidized Bacillus anthracis CoADR-RHD
3ics	Crystal structure of partially reduced Bacillus anthracis CoADR-RHD
3ict	Crystal structure of reduced Bacillus anthracis CoADR-RHD
3ilm	Crystal Structure of the Alr3790 protein from Anabaena sp. Northeast Structural Genomics Consortium Target NsR437h
3ipo	Crystal structure of YnjE
3ipp	crystal structure of sulfur-free YnjE
3iwh	Crystal Structure of Rhodanese-like Domain Protein from Staphylococcus aureus
3k9r	X-ray structure of the Rhodanese-like domain of the Alr3790 protein from Anabaena sp. Northeast Structural Genomics Consortium Target NsR437c.
3mzz	Crystal Structure of Rhodanese-like Domain Protein from Staphylococcus aureus
3nhv	Crystal Structure of BH2092 protein from Bacillus halodurans, Northeast Structural Genomics Consortium Target BhR228F
3nt6	Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase C43S/C531S Double Mutant
3nta	Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase
3ntd	Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase C531S Mutant
3o3w	Crystal Structure of BH2092 protein (residues 14-131) from Bacillus halodurans, Northeast Structural Genomics Consortium Target BhR228A
3olh	Human 3-mercaptopyruvate sulfurtransferase
3op3	Crystal Structure of Cell Division Cycle 25C Protein Isoform A from Homo sapiens
3p3a	Crystal structure of a putative thiosulfate sulfurtransferase from Mycobacterium thermoresistible
3r2u	2.1 Angstrom Resolution Crystal Structure of Metallo-beta-lactamase from Staphylococcus aureus subsp. aureus COL
3tg1	Crystal structure of p38alpha in complex with a MAPK docking partner
3tg3	Crystal structure of the MAPK binding domain of MKP7
3tp9	Crystal structure of Alicyclobacillus acidocaldarius protein with beta-lactamase and rhodanese domains
3utn	Crystal structure of Tum1 protein from Saccharomyces cerevisiae
4f67	Three dimensional structure of the double mutant of UPF0176 protein lpg2838 from Legionella pneumophila at the resolution 1.8A, Northeast Structural Genomics Consortium (NESG) Target LgR82
4jgt	Structure and kinetic analysis of H2S production by human Mercaptopyruvate Sulfurtransferase
4ocg	4OCG
4wh7	4WH7
4wh9	4WH9
5hbl	5HBL
5hbo	5HBO
5hbp	5HBP
5hbq	5HBQ
5lam	5LAM
5lao	5LAO

Links (links to other resources describing this domain)

• INTERPRO: IPR001763