The domain within your query sequence starts at position 1 and ends at position 177; the E-value for the TOP4c domain shown below is 4.06e-6.

Some of the required catalytic sites were not detected in this domain. It is probably inactive! Check the literature (PubMed 97422287 ) for details.

Catalytic residues
PositionAmino acidPresent?


DNA Topoisomerase IV
SMART accession number:SM00434
Description: Bacterial DNA topoisomerase IV, GyrA, ParC
Interpro abstract (IPR002205):

DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single- or double-strand breaks, crossing the strands through one another, then resealing the breaks [ (PUBMED:7770916) ]. These enzymes have several functions: to remove DNA supercoils during transcription and DNA replication; for strand breakage during recombination; for chromosome condensation; and to disentangle intertwined DNA during mitosis [ (PUBMED:12042765) (PUBMED:11395412) ]. DNA topoisomerases are divided into two classes: type I enzymes ( EC ; topoisomerases I, III and V) break single-strand DNA, and type II enzymes ( EC ; topoisomerases II, IV and VI) break double-strand DNA [ (PUBMED:12596227) ].

Type II topoisomerases are ATP-dependent enzymes, and can be subdivided according to their structure and reaction mechanisms: type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as for introducing both negative and positive supercoils [ (PUBMED:7980433) ].

Type IIA topoisomerases together manage chromosome integrity and topology in cells. Topoisomerase II (called gyrase in bacteria) primarily introduces negative supercoils into DNA. In bacteria, topoisomerase II consists of two polypeptide subunits, gyrA and gyrB, which form a heterotetramer: (BA)2. In most eukaryotes, topoisomerase II consists of a single polypeptide, where the N- and C-terminal regions correspond to gyrB and gyrA, respectively; this topoisomerase II forms a homodimer that is equivalent to the bacterial heterotetramer. There are four functional domains in topoisomerase II: domain 1 (N-terminal of gyrB) is an ATPase, domain 2 (C-terminal of gyrB) is responsible for subunit interactions (differs between eukaryotic and bacterial enzymes), domain 3 (N-terminal of gyrA) is responsible for the breaking-rejoining function through its capacity to form protein-DNA bridges, and domain 4 (C-terminal of gyrA) is able to non-specifically bind DNA [ (PUBMED:8982450) ].

Topoisomerase IV primarily decatenates DNA and relaxes positive supercoils, which is important in bacteria, where the circular chromosome becomes catenated, or linked, during replication [ (PUBMED:16023670) ]. Topoisomerase IV consists of two polypeptide subunits, parE and parC, where parC is homologous to gyrA and parE is homologous to gyrB.

This entry represents subunit A (gyrA and parC) of bacterial gyrase and topoisomerase IV, and the equivalent C-terminal region in eukaryotic topoisomerase II composed of a single polypeptide. This subunit has DNA-binding capacity.

GO process:DNA topological change (GO:0006265)
GO function:DNA binding (GO:0003677), DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity (GO:0003918), ATP binding (GO:0005524)
Family alignment:
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There are 40904 TOP4c domains in 40879 proteins in SMART's nrdb database.

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